Colossal Biosciences has announced that its de-extinct dire wolves—Romulus, Remus, and Khaleesi—are now breeding-aged and the firm plans to expand the pack later this year. The development marks a significant step for the Texas-based company in its mission to restore extinct species through genetic engineering.
The dire wolf pups, born in late 2024 and early 2025, represent the world’s first de-extinct animals. They have thrived in a secure 2,000-acre preserve, reaching milestones like learning to process whole deer carcasses and now showing readiness for natural breeding behaviors.
“The dire wolf pack is actually breeding-aged at this point,” Matt James, Colossal’s chief animal officer, said, adding “But we will initially grow the pack through assisted reproduction while we create new, genetically diverse individuals.”
The company intends to engineer two to four additional pups to boost genetic diversity before allowing full natural breeding. “The plan is to create an inter-breedable population of dire wolves in which they would eventually breed naturally to create a sustainable population of the world’s first de-extinct species,” James continued.
He further added, “We will grow the population through assisted reproduction initially and then eventually only rely on natural breeding.”
“The dire wolves are doing great,” Ben Lamm, Colossal’s CEO and co-founder, stated., adding “The three dire wolves live on a 2,000-acre secure, expansive ecological preserve that allows us to monitor and manage them while providing them a semi-wild habitat to thrive in. We hope to have more dire wolf pups by the end of the year.”
Colossal reconstructed the dire wolf genome from ancient DNA fragments in bone samples, including a 72,000-year-old skull. Scientists then edited gray wolf embryos to incorporate key traits: a white coat, larger teeth, more muscular build, and distinctive howl. Embryos were implanted in surrogate dogs, with births by caesarean section.
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